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normal sheep igg  (R&D Systems)


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    R&D Systems normal sheep igg
    Normal Sheep Igg, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 49 article reviews
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    93/100 stars

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    Fig. 1. CDH6 protein is detected in the developing mouse cerebral cortex. (A–G) Immunostaining for CDH6 (green) and DAPI (magenta) using sections from the cerebral cortex at E12.0 (A) (n = 6), E14.0 (B) (n = 6), E16.0 (C) (n = 4) and E18.0 (D–F) (n = 10) of the ICR mouse. The boxed regions in (D) are shown at higher magnification in (E) and (F). (G) Control staining with <t>normal</t> <t>sheep</t> <t>IgG</t> instead of CDH6 <t>antibody</t> (n = 10). The lower panels in (A–G) show grayscale, single-channel images of CDH6 (A–F) and normal sheep IgG (G), respectively. (H–J) The cerebral cortices (E17.0) that had been electroporated with CAG-EGFP plasmid on E14.0 were stained for DCX (H, I) (n = 4) and Nestin (NES) (n = 4) (J). Arrows in (H) and (I) indicate colocalization of immunosignals of CDH6/DCX in EGFP-positive neurons in CP and IZ, respectively. Cell outline is highlighted using the GFP signal as a guide in the CDH6 and DCX panels (magenta lines in H and I). The second panels from the left in (H–J) show CDH6 grayscale, single-channel images. The fourth panels from the left in (H–I) show DCX grayscale, single-channel images. The fourth panel from the left in (J) shows a NES grayscale, single-channel image. (K–M) Immunostaining for HA (green) and DAPI (magenta) using sections from the cerebral cortex at P0 of the Cdh6-HA KI mouse (n = 4). Boxed regions in (K) are shown at higher magnification in (L) and (M). (N) Control staining with normal rat IgG instead of HA antibody (n = 4). The lower panels in (K–N) show grayscale, single-channel images of CDH6 (K–M) and normal rat IgG (N), respectively. Scale bars: 100 lm in (A, B, C, D, G, K, N); 40 lm in (E, F, L, M); 5 lm in (H, I); 20 lm in (J). CP, cortical plate; DCX, Doublecortin; HP, hippocampus; IZ, intermediate zone; MAZ, multipolar cell accumulation zone; MZ, marginal zone; Ncx, neocortex; NES, Nestin; SLM, stratum lacunosum moleculare; SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum; VZ, ventricular zone.
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    Fig. 1. CDH6 protein is detected in the developing mouse cerebral cortex. (A–G) Immunostaining for CDH6 (green) and DAPI (magenta) using sections from the cerebral cortex at E12.0 (A) (n = 6), E14.0 (B) (n = 6), E16.0 (C) (n = 4) and E18.0 (D–F) (n = 10) of the ICR mouse. The boxed regions in (D) are shown at higher magnification in (E) and (F). (G) Control staining with <t>normal</t> <t>sheep</t> <t>IgG</t> instead of CDH6 <t>antibody</t> (n = 10). The lower panels in (A–G) show grayscale, single-channel images of CDH6 (A–F) and normal sheep IgG (G), respectively. (H–J) The cerebral cortices (E17.0) that had been electroporated with CAG-EGFP plasmid on E14.0 were stained for DCX (H, I) (n = 4) and Nestin (NES) (n = 4) (J). Arrows in (H) and (I) indicate colocalization of immunosignals of CDH6/DCX in EGFP-positive neurons in CP and IZ, respectively. Cell outline is highlighted using the GFP signal as a guide in the CDH6 and DCX panels (magenta lines in H and I). The second panels from the left in (H–J) show CDH6 grayscale, single-channel images. The fourth panels from the left in (H–I) show DCX grayscale, single-channel images. The fourth panel from the left in (J) shows a NES grayscale, single-channel image. (K–M) Immunostaining for HA (green) and DAPI (magenta) using sections from the cerebral cortex at P0 of the Cdh6-HA KI mouse (n = 4). Boxed regions in (K) are shown at higher magnification in (L) and (M). (N) Control staining with normal rat IgG instead of HA antibody (n = 4). The lower panels in (K–N) show grayscale, single-channel images of CDH6 (K–M) and normal rat IgG (N), respectively. Scale bars: 100 lm in (A, B, C, D, G, K, N); 40 lm in (E, F, L, M); 5 lm in (H, I); 20 lm in (J). CP, cortical plate; DCX, Doublecortin; HP, hippocampus; IZ, intermediate zone; MAZ, multipolar cell accumulation zone; MZ, marginal zone; Ncx, neocortex; NES, Nestin; SLM, stratum lacunosum moleculare; SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum; VZ, ventricular zone.
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    Fig. 1. CDH6 protein is detected in the developing mouse cerebral cortex. (A–G) Immunostaining for CDH6 (green) and DAPI (magenta) using sections from the cerebral cortex at E12.0 (A) (n = 6), E14.0 (B) (n = 6), E16.0 (C) (n = 4) and E18.0 (D–F) (n = 10) of the ICR mouse. The boxed regions in (D) are shown at higher magnification in (E) and (F). (G) Control staining with <t>normal</t> <t>sheep</t> <t>IgG</t> instead of CDH6 <t>antibody</t> (n = 10). The lower panels in (A–G) show grayscale, single-channel images of CDH6 (A–F) and normal sheep IgG (G), respectively. (H–J) The cerebral cortices (E17.0) that had been electroporated with CAG-EGFP plasmid on E14.0 were stained for DCX (H, I) (n = 4) and Nestin (NES) (n = 4) (J). Arrows in (H) and (I) indicate colocalization of immunosignals of CDH6/DCX in EGFP-positive neurons in CP and IZ, respectively. Cell outline is highlighted using the GFP signal as a guide in the CDH6 and DCX panels (magenta lines in H and I). The second panels from the left in (H–J) show CDH6 grayscale, single-channel images. The fourth panels from the left in (H–I) show DCX grayscale, single-channel images. The fourth panel from the left in (J) shows a NES grayscale, single-channel image. (K–M) Immunostaining for HA (green) and DAPI (magenta) using sections from the cerebral cortex at P0 of the Cdh6-HA KI mouse (n = 4). Boxed regions in (K) are shown at higher magnification in (L) and (M). (N) Control staining with normal rat IgG instead of HA antibody (n = 4). The lower panels in (K–N) show grayscale, single-channel images of CDH6 (K–M) and normal rat IgG (N), respectively. Scale bars: 100 lm in (A, B, C, D, G, K, N); 40 lm in (E, F, L, M); 5 lm in (H, I); 20 lm in (J). CP, cortical plate; DCX, Doublecortin; HP, hippocampus; IZ, intermediate zone; MAZ, multipolar cell accumulation zone; MZ, marginal zone; Ncx, neocortex; NES, Nestin; SLM, stratum lacunosum moleculare; SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum; VZ, ventricular zone.
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    Fig. 1. CDH6 protein is detected in the developing mouse cerebral cortex. (A–G) Immunostaining for CDH6 (green) and DAPI (magenta) using sections from the cerebral cortex at E12.0 (A) (n = 6), E14.0 (B) (n = 6), E16.0 (C) (n = 4) and E18.0 (D–F) (n = 10) of the ICR mouse. The boxed regions in (D) are shown at higher magnification in (E) and (F). (G) Control staining with <t>normal</t> <t>sheep</t> <t>IgG</t> instead of CDH6 <t>antibody</t> (n = 10). The lower panels in (A–G) show grayscale, single-channel images of CDH6 (A–F) and normal sheep IgG (G), respectively. (H–J) The cerebral cortices (E17.0) that had been electroporated with CAG-EGFP plasmid on E14.0 were stained for DCX (H, I) (n = 4) and Nestin (NES) (n = 4) (J). Arrows in (H) and (I) indicate colocalization of immunosignals of CDH6/DCX in EGFP-positive neurons in CP and IZ, respectively. Cell outline is highlighted using the GFP signal as a guide in the CDH6 and DCX panels (magenta lines in H and I). The second panels from the left in (H–J) show CDH6 grayscale, single-channel images. The fourth panels from the left in (H–I) show DCX grayscale, single-channel images. The fourth panel from the left in (J) shows a NES grayscale, single-channel image. (K–M) Immunostaining for HA (green) and DAPI (magenta) using sections from the cerebral cortex at P0 of the Cdh6-HA KI mouse (n = 4). Boxed regions in (K) are shown at higher magnification in (L) and (M). (N) Control staining with normal rat IgG instead of HA antibody (n = 4). The lower panels in (K–N) show grayscale, single-channel images of CDH6 (K–M) and normal rat IgG (N), respectively. Scale bars: 100 lm in (A, B, C, D, G, K, N); 40 lm in (E, F, L, M); 5 lm in (H, I); 20 lm in (J). CP, cortical plate; DCX, Doublecortin; HP, hippocampus; IZ, intermediate zone; MAZ, multipolar cell accumulation zone; MZ, marginal zone; Ncx, neocortex; NES, Nestin; SLM, stratum lacunosum moleculare; SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum; VZ, ventricular zone.
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    Fig. 1. CDH6 protein is detected in the developing mouse cerebral cortex. (A–G) Immunostaining for CDH6 (green) and DAPI (magenta) using sections from the cerebral cortex at E12.0 (A) (n = 6), E14.0 (B) (n = 6), E16.0 (C) (n = 4) and E18.0 (D–F) (n = 10) of the ICR mouse. The boxed regions in (D) are shown at higher magnification in (E) and (F). (G) Control staining with <t>normal</t> <t>sheep</t> <t>IgG</t> instead of CDH6 <t>antibody</t> (n = 10). The lower panels in (A–G) show grayscale, single-channel images of CDH6 (A–F) and normal sheep IgG (G), respectively. (H–J) The cerebral cortices (E17.0) that had been electroporated with CAG-EGFP plasmid on E14.0 were stained for DCX (H, I) (n = 4) and Nestin (NES) (n = 4) (J). Arrows in (H) and (I) indicate colocalization of immunosignals of CDH6/DCX in EGFP-positive neurons in CP and IZ, respectively. Cell outline is highlighted using the GFP signal as a guide in the CDH6 and DCX panels (magenta lines in H and I). The second panels from the left in (H–J) show CDH6 grayscale, single-channel images. The fourth panels from the left in (H–I) show DCX grayscale, single-channel images. The fourth panel from the left in (J) shows a NES grayscale, single-channel image. (K–M) Immunostaining for HA (green) and DAPI (magenta) using sections from the cerebral cortex at P0 of the Cdh6-HA KI mouse (n = 4). Boxed regions in (K) are shown at higher magnification in (L) and (M). (N) Control staining with normal rat IgG instead of HA antibody (n = 4). The lower panels in (K–N) show grayscale, single-channel images of CDH6 (K–M) and normal rat IgG (N), respectively. Scale bars: 100 lm in (A, B, C, D, G, K, N); 40 lm in (E, F, L, M); 5 lm in (H, I); 20 lm in (J). CP, cortical plate; DCX, Doublecortin; HP, hippocampus; IZ, intermediate zone; MAZ, multipolar cell accumulation zone; MZ, marginal zone; Ncx, neocortex; NES, Nestin; SLM, stratum lacunosum moleculare; SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum; VZ, ventricular zone.
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    Fig. 1. CDH6 protein is detected in the developing mouse cerebral cortex. (A–G) Immunostaining for CDH6 (green) and DAPI (magenta) using sections from the cerebral cortex at E12.0 (A) (n = 6), E14.0 (B) (n = 6), E16.0 (C) (n = 4) and E18.0 (D–F) (n = 10) of the ICR mouse. The boxed regions in (D) are shown at higher magnification in (E) and (F). (G) Control staining with normal sheep IgG instead of CDH6 antibody (n = 10). The lower panels in (A–G) show grayscale, single-channel images of CDH6 (A–F) and normal sheep IgG (G), respectively. (H–J) The cerebral cortices (E17.0) that had been electroporated with CAG-EGFP plasmid on E14.0 were stained for DCX (H, I) (n = 4) and Nestin (NES) (n = 4) (J). Arrows in (H) and (I) indicate colocalization of immunosignals of CDH6/DCX in EGFP-positive neurons in CP and IZ, respectively. Cell outline is highlighted using the GFP signal as a guide in the CDH6 and DCX panels (magenta lines in H and I). The second panels from the left in (H–J) show CDH6 grayscale, single-channel images. The fourth panels from the left in (H–I) show DCX grayscale, single-channel images. The fourth panel from the left in (J) shows a NES grayscale, single-channel image. (K–M) Immunostaining for HA (green) and DAPI (magenta) using sections from the cerebral cortex at P0 of the Cdh6-HA KI mouse (n = 4). Boxed regions in (K) are shown at higher magnification in (L) and (M). (N) Control staining with normal rat IgG instead of HA antibody (n = 4). The lower panels in (K–N) show grayscale, single-channel images of CDH6 (K–M) and normal rat IgG (N), respectively. Scale bars: 100 lm in (A, B, C, D, G, K, N); 40 lm in (E, F, L, M); 5 lm in (H, I); 20 lm in (J). CP, cortical plate; DCX, Doublecortin; HP, hippocampus; IZ, intermediate zone; MAZ, multipolar cell accumulation zone; MZ, marginal zone; Ncx, neocortex; NES, Nestin; SLM, stratum lacunosum moleculare; SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum; VZ, ventricular zone.

    Journal: The FEBS journal

    Article Title: Cadherin-6 controls neuronal migration during mouse neocortical development via an integrin-mediated pathway.

    doi: 10.1111/febs.70150

    Figure Lengend Snippet: Fig. 1. CDH6 protein is detected in the developing mouse cerebral cortex. (A–G) Immunostaining for CDH6 (green) and DAPI (magenta) using sections from the cerebral cortex at E12.0 (A) (n = 6), E14.0 (B) (n = 6), E16.0 (C) (n = 4) and E18.0 (D–F) (n = 10) of the ICR mouse. The boxed regions in (D) are shown at higher magnification in (E) and (F). (G) Control staining with normal sheep IgG instead of CDH6 antibody (n = 10). The lower panels in (A–G) show grayscale, single-channel images of CDH6 (A–F) and normal sheep IgG (G), respectively. (H–J) The cerebral cortices (E17.0) that had been electroporated with CAG-EGFP plasmid on E14.0 were stained for DCX (H, I) (n = 4) and Nestin (NES) (n = 4) (J). Arrows in (H) and (I) indicate colocalization of immunosignals of CDH6/DCX in EGFP-positive neurons in CP and IZ, respectively. Cell outline is highlighted using the GFP signal as a guide in the CDH6 and DCX panels (magenta lines in H and I). The second panels from the left in (H–J) show CDH6 grayscale, single-channel images. The fourth panels from the left in (H–I) show DCX grayscale, single-channel images. The fourth panel from the left in (J) shows a NES grayscale, single-channel image. (K–M) Immunostaining for HA (green) and DAPI (magenta) using sections from the cerebral cortex at P0 of the Cdh6-HA KI mouse (n = 4). Boxed regions in (K) are shown at higher magnification in (L) and (M). (N) Control staining with normal rat IgG instead of HA antibody (n = 4). The lower panels in (K–N) show grayscale, single-channel images of CDH6 (K–M) and normal rat IgG (N), respectively. Scale bars: 100 lm in (A, B, C, D, G, K, N); 40 lm in (E, F, L, M); 5 lm in (H, I); 20 lm in (J). CP, cortical plate; DCX, Doublecortin; HP, hippocampus; IZ, intermediate zone; MAZ, multipolar cell accumulation zone; MZ, marginal zone; Ncx, neocortex; NES, Nestin; SLM, stratum lacunosum moleculare; SO, stratum oriens; SP, stratum pyramidale; SR, stratum radiatum; VZ, ventricular zone.

    Article Snippet: For control staining for CDH6 and HA, normal sheep IgG (ChromPure sheep IgG, whole molecule; Jackson ImmunoResearch Laboratories, cat# 013-000-003) and normal rat IgG (ChromPure rat IgG, whole molecule; Jackson ImmunoResearch Laboratories, cat# 102-000-003) were used, respectively.

    Techniques: Immunostaining, Control, Staining, Plasmid Preparation